Zinc Finger Proteins: Methods and Protocols

Part I

Chapter 1 PRDM14, a zinc finger protein, regulates cancer stemness

Chapter 2 Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins

Chapter 3 Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation

Chapter 4 Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology

Chapter 5 Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers

Chapter 6 Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins

Chapter 7 Directed evolution of targeted recombinases for genome engineering

Chapter 9 Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins

Chapter 10 Genome editing of MSCs as a platform for cell therapy

Part II

Chapter 11 Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells

Chapter 12 Selection and characterization of DNA aptamers against FokI nuclease domain

Chapter 13 An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs

Part III

Chapter 14 Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering

Chapter 15 Gene editing in channel catfish via double electroporation of zinc finger nucleases

Chapter 16 Delivery of mtZFNs into early mouse embryos

Chapter 17 Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain

Chapter 19 Manufacturing and delivering genome-editing proteins