Zymography: Methods and Protocols

1. Zymography Principles

Jeff Wilkesman and Liliana Kurz

Part II: Endopeptidase Zymography

2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

Hidetaro Yasumitsu

3. Cysteine Protease Zymography: Brief Review

Jeff Wilkesman

4. Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

Gavin Kernaghan and Michael Mayerhofer

5. Detection of Aspartic Proteinase Activities Using Gel Zymography

Handunge Kumudu Irani Perera

P?ter Bencsik, Monika Bartekova, Anik? G?rbe, Krisztina Kiss, J?nos P?l?czi, Jana Radosinska, Gergő Szűcs, and P?ter Ferdinandy

7. Characterization of Novel Collagenolytic Proteases

Goran Mucic, Brankica Raskovic, and Natalija Polovic

8. Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors

9. Detection and Characterization of Bacterial Proteinases Using Zymography

Madathiparambil G. Madanan and Ambili Mechoor

10. A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography

Shivcharan Prasad and Ipsita Roy

11. Reverse Zymography: Overview and Pitfalls

Kanika Sharma and Debasish Bhattacharyya

12. Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions

Aastha Chhabra and Vibha Rani

Part IV: 2D Zymography

13. Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography

Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, and Zezong Gu

14. Two-Dimensional Zymography of Proteases from Steatotic Duck Liver

Part V: Special Zymography Cases

15. Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases after SDS-PAGE and Blotting

Silvia Ravera and Isabella Panfoli

16. Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel